E39 should be used in parallel with this nonselective formula. After the mixture is a bit bubbly, kill the heat and add the vanilla extract. A highly nutritious medium used for the isolation and cultivation of fastidious microorganisms, especially neisseria and haemophillus species from a variety of clinical specimens. One of the unique qualities of an agar gel is hysteresis, meaning theres a large differential between agars setting and melting temperature 95f33c and 175f80c respectively. Chocolate agar gc base with hemoglobin and supplements for. Principles and methods of sterilization in health sciencies, 2nd ed. Chocolate agar is an enriched medium, thus nonpathogenic organisms may overgrow pathogenic bacteria. Agar production by modern techniques of industrial freezing was initiated in california. Chocolate agar choc or chocolate blood agar cba, is a nonselective, enriched growth medium used for isolation of pathogenic bacteria. Add in the agar agar powder and stir until completely dissolved. Types of bacteria on blood and chocolate agar and the. The name is itself derived from the fact that red blood cell rbc lysis gives the medium a chocolatebrown color. Product information and quality control sheet chocolate agar intended use chocolate agar is an enriched medium for the isolation and cultivation of fastidious microorganisms, especially neisseria and haemophilus species from a variety of clinical specimens.
Our chocolate agar enriched should be stored away from direct light at 4 c to 8 c. In another saucepan add a cup of milk, half cup of sugar, add about 5 tbsp of cocoa powder and half cup of melted chocolate to the milk. Bbl chocolate ii agar gc ii agar with hemoglobin and. If a delay in and haemophilus species from a variety of clinical specimens. The study demonstrated that reactions occurring on chocolate agar are useful in identifying grampositive cocci. The medium side should be uppermost to prevent excessive accumulation of moisture on the agar surface. Suitability of a chocolate agar based medium for the transportation and long term storage of neisseria gonorrhoeae isolates at room temperature sidharath d. In addition, if the specimen has been obtained from a neonate among whom e. A gelatinous material derived from certain marine algae. Bacillus anthracis colonial morphology, depicted in the image below, is found when grown on a medium of chocolate agar, for a 24 hour time period, at a.
Under these conditions this medium has a shelf life of 10 weeks from the date of manufacture. The centrifuged deposit should be inoculated on chocolate agar and incubated in a humidified atmosphere and 10%co 2, and on blood agar incubated aerobically. Jul 10, 20 agar agar is a vegan substitute for gelatin. Streptococcus pneumoniae y haemophilus influenzae salud. Traditional gelatin is made with powdered cow hooves. Get a printable copy pdf file of the complete article 1.
Suitable for the isolation of most pathogens including the most fastidious and is particularly useful for the cultivation of haemophilus spp. It is a variant of the blood agar plate, containing red blood cells that have been lysed by slowly heating to 80c. Our chocolate agar modified martinlewis should be stored away from direct light at 4c to 8 c. Intended use chocolate agar is an enriched medium for the isolation and cultivation of fastidious microorganisms, especially neisseria inoculated promptly after specimen collection. This document was uploaded by user and they confirmed that they have.
Learn vocabulary, terms, and more with flashcards, games, and other study tools. Chocolate agar definition of chocolate agar by medical. Colonies on chocolate agar are pinkishbrown and translucent, exhibit smooth consistency and defined margins, and are typically 0. To prepare chocolate agar base, see instructions for use for criterion hemoglobin powder, which is prepared separately and added to gc agar base along with necessary supplements andor antibiotics. Haemophilus are small, pleomorphic, gramnegative coccobacilli. Chocolate agar article about chocolate agar by the free. Sterilize by autoclaving at 15 lbs pressure 121c for 15 minutes. Manual of bbl products and laboratory procedures, 6th ed. Add in the agaragar powder and stir until completely dissolved. Chocolate ii agar is an enriched medium for the isolation and cultivation of neisseria species. Chemtrec usa 8004249300 safety data sheet r00 supplier oxoid limited wade road basingstoke hants rg24 8pw. It is a variant of the blood agar plate, containing red blood cells that have been lysed by slowly heating to 80 c. Include plates of a previously tested lot of chocolate ii agar as controls for all strains.
Download as docx, pdf, txt or read online from scribd. Criterion gc agar base for the preparation of chocolate agar. The basic formula for agaragar powder is 1 12 teaspoons of powder for every 2 cups of liquidheated until boiling to dissolve the agaragarfor a firmbutnotrubbery texture. Full text get a printable copy pdf file of the complete article 434k, or click on a page image below to browse page by page. The treatment and control of gonorrhea is complicated by increasing resistance to a variety of antimicrobial agents, including penicillin, tetracycline, and fluoroquinolones. Colour and clarity of prepared medium basal medium. Pdf suitability of a chocolate agarbased medium for the. Chocolate agar, a differential medium for grampositive cocci. This makes it possible to serve a warm gel using agar, something that isnt possible with traditional gelatin based gels. Suitability of a chocolate agarbased medium for the transportation and long term storage of neisseria gonorrhoeae isolates at room temperature sidharath d. Chocolate agar is used for the isolation of fastidious organisms, such as haemophilus influenzae, when incubated at. Chocolate agar also contains red blood cells, but with chocolate agar, the blood is autoclaved, thus changing the red blood cells to a brown color. The media is further enriched with suplex polyvitex that provides vitamins, amino acids, coenzymes, glucose and other factors which improve the growth of neisseria and haemophillus species. Chocolate agar base is recommended for the isolation of neisseria gonorrhoeae from chronic and acute cases of gonococcal infections.
Summary and explanation chocolate agar was formulated in 1927 using peptones and yeast extract. Summary and explanation bacto gc medium base was designed in 1947. Chocolate agar base, with the addition of supplements, gives excellent growth of the gonococcus without overgrowth by contaminating organisms. A japanese legend is told about the first preparation of agar. Chocolate agar is a nonselective, enriched growth medium. Chocolate ii agar gc ii agar with hemoglobin and isovitalex for the isolation and cultivation of fastidious microorganisms, especially neisseria and haemophilus species, from a variety of clinical specimens. Aug 20, 2012 bacillus anthracis colonial morphology, depicted in the image below, is found when grown on a medium of chocolate agar, for a 24 hour time period, at a temperature of 37 c. The gc base contains nitrogenous nutrients in the form of casein and meat peptones, phosphate buffer to maintain ph and corn starch, which neutralizes toxic fatty acids that may be present in the agar. Under these conditions this medium has a shelf life of 8 weeks from the date of manufacture. Full text full text is available as a scanned copy of the original print version. This medium can be made selective by the addition of antibiotics. The medium is often used to observate the forms of haemolysis from pathogenic microorganisms. Chocolate agar intended use remel chocolate agar is a solid medium recommended for use in qualitative procedures for the isolation and cultivation of fastidious organisms, especially neisseria and haemophilus species. Chocolate agar is used for the isolation of fastidious organisms, such as haemophilus influenzae, when incubated at 3537c in a 5% co2 atmosphere.
Under these conditions this medium has a shelf life of 8 weeks from the date of. Chocolate brown coloured, opaque gel forms in petri plates reaction reaction of 4. This organism is oxidasepositive, and growth is best obtained on chocolate agar or thayer martin agar. Chocolate agar is used for growing fastidious respiratory bacteria, such as haemophilus influenzae and neisseria meningitidis. Agar sangre agar chocolate by laura garcia on prezi. As with traditional gelatin, this vegan substitute has a neutral flavor and does not taste like seaweed. Chocolate agar manufacturer trade name chocolate agar product no.
234 977 1 577 1227 37 1414 991 557 558 759 1297 664 1283 763 1303 650 1552 1454 774 1138 269 856 506 219 932 1320 76 686 1047 1417 172 820 1168 1004 742 1273 297 834 95 259