The six rhizobium species and their more common hosts were as follows. Effect of laser leveling, water quantities and rhizobium. Pdf on feb 10, 20, solomon legesse and others published symbiotic and phenotypic characteristics of rhizobia nodulating faba bean vicia faba from tahtay koraro, northwestern zone of tigray. Rhizobium leguminosarum biovar viciae, bradyrhizobium sp. Vicia faba, also known in the culinary sense as the broad bean, fava bean, or faba bean, is a species of flowering plant in the pea and bean family fabaceae. Dec 12, 2001 firstly, this study investigated potential differences between rhizobium leguminosarum bv.
Effect of nitrogen and sulphur application and seed. On the other hand, few studies have been undertaken in order to compare mutagenic agents for their ability to induce useful genetic variability in vicia faba l. Rhizobium leguminosarum inoculation and the decrease in damage of faba bean vicia faba infected with broad beanmottle bromovirus and bean yellow mosaic potyvirus. These isolates were evaluated with two reference strains psra and psrb of rhizobium leguminosarum for. We isolated 65 rhizobial strains from faba bean vicia faba l. The quantification of nitrogen fixation by vicia faba inoculated with rhizobium leguminosarum in relation to micronutrient application was assessed in pot experiments.
Background and aims the aim of this study was to investigate the effects of the interactions between the microbial symbionts, rhizobium and arbuscular mycorrhizal fungi amf on n and p accumulation by broad bean vicia faba and how increased n and p content influence biomass production, leaf area and net photosynthetic rate. Low soil phosphorus availability is a major constraint for crops especially when they are depending on symbiotic nitrogen fixation. Characterization and evaluation of vicia faba for some plant. Here we screened rhizobial strains isolated from nodules of faba bean v. Comparative study for irrigation with nile water and wastewater ahmed a. Contrasting rhizobium inoculation requirements of zero. It has been reported that legumes inoculated with rhizobium species and supplemented with p. Pdf rhizobium strains from nodules of vicia faba were diverse in plasmid content and serology. Interactions between rhizobium, antagonistic bacteria and. Rhizobia are special bacteria that can live in the soil or in nodules formed on the roots of legumes. Faba bean contains high protein 24% and appreciable amount of minerals and vitamins, and thus it is part of the various popular ethiopian dishes. Rhizobium strains from nodules of vicia faba were diverse in plasmid content and serology.
Nitrogen was applied at flowering, whereas sulphur was applied as foliar applications in elemental form, several times during the growth cycle of plants or only once at four different growth stages. Varieties with smaller, harder seeds that are fed to horses or other animals are called field bean, tic bean or tick bean. The obtained dendogram indicated that the isolates belonging to rhizobium leguminosarum biovar viciaen. Therefore, understanding the impact of rhizobial inoculation and contrasting soil rhizobia on nodulation and n2 fixation in faba bean is crucial to optimize the crop yield, particularly under low fertility soil conditions. Characterization and evaluation of vicia faba for some.
Hence, ten isolates were isolated using plantsoil trap method from soil samples collected from farm lands. The influence of rhizobium and arbuscular mycorrhizal fungi. Eight distinct sublineages of rhizobium leguminosarum bv. Variability among rhizobium strains originating from nodules of vicia faba article pdf available in applied and environmental microbiology 617. Contrasting rhizobium inoculation requirements of zerotannin. The level and mechanisms of population mixing among faba bean vicia faba rhizobia of different geographic origins three ecoregions of china and several western countries were analysed by sequencing three chromosomal housekeeping loci atpd, reca and glnii and one nodulation gene nodd. The evaluation of rhizobium leguminosarum strains effectiveness in field beans vicia faba l. These two strains similarly colonized the roots of both hosts. Comparative study for irrigation with nile water and wastewater. Vicia faba was an important legume food crop in ancient civilizations until the introduction of phaseolus species from the americas. The effect of nitrogen and sulphur application and seed inoculation on the yield of beans vicia faba was studied at two locations in 197980 and 19801. Influence of rhizobium and arbuscular mycorrhizal fungi on. What rhizobia inoculates what crop advanced biological.
Characterization of phosphatesolubilizing rhizobia from. Effect of phosphorus application and time of harvest on the seed yield and quality of faba bean. In addition to evaluate effect of rhizobium isolates on in vivo growth of faba bean vicia faba l infected with. The 16s rrna gene sequences were identical to that of r. Introduction to rhizobia summary this module introduces the general role of microorganisms in the soil, and specifically the rhizobia. Nodulation of lupinus albus and vicia faba by rhizobium. Rhizobium leguminosarum cytochromes vicia faba springerlink.
The nitrogen fixing potential of vicia faba rhizobia r. Effects of rhizobium inoculation and supplementation with. Pdf symbiotic and phenotypic characteristics of rhizobia. Contrasting rhizobium inoculation requirements of zerotannin faba bean and narrowleafed lupin in western canada. The balance calculations showed nitrogen gains up to 260 and 478 mg npot respectively for soaking and foliar spray techniques using a mixture of the micronutrients mo, mn, fe. Thirtyone nodulating rhizobium strains were collected from root nodules of spring and winter type faba bean cultivars grown in micro ecoarea, i. Variability among rhizobium strains originating from nodules of. Pdf improvement of some growth and yield parameters of. Influence of rhizobiumazotobacter and rhizobiumazospirillum. Lentils, vetches and faba beans austrian winter pea, chickling vetch, faba bean, field pea, flat pea, garden pea, hairy vetch. Currently, wide varieties of commercial legume inoculants have been produced and have been tested and therefore are available for use in different part of the world.
Faba bean makes a significant contribution to soil fertility restoration as a suitable rotation crop that fixes. Seeds were inoculated with rhizobium and planted at different leveling slopes zero, 0. Ricciardi, in genetic improvement of vegetable crops, 1993. In ethiopia, grain crops are grown annually on approximately 12. Bacteroids of the effective strain contained cytochromes c, b and some coreactive pigments which remained incompletely identified. Effectiveness of native rhizobium on nodulation and yield of faba bean vicia faba l. Symbiotic nitrogen fixation in faba beans springerlink.
The cytochemical effect of rhizobia on vicia faba msc thesis faculty of agric. In root nodules, they form a symbiotic association with the legume. Application of combination of rhizobium isolates to seedling before the time of pathogen inoculation gave the highest 88. However, the biocontrol agents posed increased shoot height, shoot weight, root length, and lesser damage of flower number to fusarium solani inoculated plants. Several fastgrowing strains nodulating vicia faba in peru, spain and tunisia formed a cluster related to rhizobium leguminosarum. After 40 days of incubation of lime, fym, and compost, mineral p was applied in the form of banding at planting. Plant rhizobium symbiosis, seed nutraceuticals, and waste. Azb department of botany and microbiology, faculty of. Effects of rhizobium inoculation and supplementation with p. To achieve this aim, 30 isolates were sampled from root nodules of faba bean in different geographical regions in morocco. Some rhizobial strains were reported to solubilize both organic and inorganic complex phosphates. The symbiotic efficiency and phylogeny of these strains were studied. Effect of nitrogen and sulphur application and seed inoculation with rhizobium leguminosarum on the yield of beans vicia faba in field trials volume 100 issue 1 j.
Advances in biology hindawi publishing corporation. Diversity of rhizobia nodulating faba bean vicia faba. The effect of rhizobia isolates against black root rot. Nodulation of lupinus albus and vicia faba by rhizobium spp. Variability among rhizobium strains originating from. Variability among rhizobium strains originating from nodules. Improvement of faba bean production in saltaffected soils. Therefore, understanding the impact of rhizobial inoculation and contrasting soil rhizobia on nodulation and n2. Effectiveness of native rhizobium on nodulation and yield of. Effectively nitrogen fixing strains were isolated from both winter type and spring type cultivars.
Results of multilocus gel electrophoresis and restriction fragment length polymorphism indicated several deep chromosomal lineages among the strains. Broad bean shows features fitting sustainable agriculture model, as it benefits from symbiosis with. Bacteroids of the partlyeffective strain contained cytochrome a a peak at 597 nm at. It covers 370,000 hectares with an annual production of 450,000 tones. Those isolates were morphologically characterized using yema medium and authenticated as nodulating rhizobia using sand culture. Pdf the evaluation of rhizobium leguminosarum strains. Integrated acid soil management for growth, nodulation, and.
The balance calculations showed nitrogen gains up to 260 and 478 mg npot respectively for soaking and foliar spray techniques using a mixture of the micronutrients mo, mn, fe and zn. Isolation and evaluation of phosphate solubilizing rhizobia. Pdf effects of rhizobium leguminosarum inoculation on. Italy is a major european producer, with 49,000 hectares devoted to this crop. Rhizobium meliloti alfalfa and bur clover, medicago spp.
The effect of rhizobium isolates on the development of radial mycelium of f. They are grouped in two main generathe fastgrowing rhizobium species and the slowgrowing bradyrhizobium species. The faba bean plants remained stunted and showed poor growth response in the absence of rhizobium isolates. In all cases, however, the increase in nodule number was accompanied by a reduction in their dry weight. Abstract nodule number per plant of lupinus albus cv. Diversity of rhizobia nodulating faba bean vicia faba growing in egypt. Pdf variability among rhizobium strains originating from. Genetic diversity and symbiotic efficiency of nodulating. The influence of rhizobium and arbuscular mycorrhizal. The experiments were carried out using the dual culture technique. Sep 07, 2014 zerotannin faba bean vicia faba minor and narrowleafed lupin lupinus angustifolius l. Interactions between rhizobium, antagonistic bacteria and fungal pathogens in faba bean shirin akhter master in biotechnology faculty of natural resources and agricultural sciences department of forest mycology and plant pathology masters thesis 30 hec second cycle, a2e biotechnology masters programme uppsala 2014. Four rhizobialike strains, isolated from root nodules of pisum sativum and vicia faba grown in anhui and jiangxi provinces of china, were grouped into the genus rhizobium but were distinct from all recognized species of the genus rhizobium by phylogenetic analysis of 16s rrna and housekeeping genes.
The size and symbiotic effectiveness, withvicia faba, ofrhizobium leguminosarum populations from five locations in southern britain has been estimated. The effect of rhizobia isolates against black root rot disease of faba bean vicia faba l caused by fusarium solani. These isolates were evaluated with two reference strains psra and psrb of rhizobium leguminosarum for their resistance to environmental. The implications of the results for improving the productivity of field beans are discussed. We conducted a 6 siteyear study to compare the effects of several commercial rhizobial. Isolation and evaluation of phosphate solubilizing.
Rhizobium alters inducible defenses in broad bean, vicia faba. Jun 17, 2004 background and aims the aim of this study was to investigate the effects of the interactions between the microbial symbionts, rhizobium and arbuscular mycorrhizal fungi amf on n and p accumulation by broad bean vicia faba and how increased n and p content influence biomass production, leaf area and net photosynthetic rate. Integrated acid soil management for growth, nodulation. Azb department of botany and microbiology, faculty of science, zagazig university, zagazig, egypt. Improvement of some growth and yield parameters of faba bean vicia faba by inoculation with rhizobium laguerreae and arbuscular mycorrhizal fungi. This study was designed to assess the effect of glyphosate and mancozeb on growth of vicia faba rhizobia isolates in vitro and on their n 2 fixation performance. Improvement of faba bean yield using rhizobiumagrobacterium. Nitrogen fixation faba bean nodule formation broad bean rhizobium. Zerotannin faba bean vicia faba minor and narrowleafed lupin lupinus angustifolius l. Influence of micronutrients on nitrogen fixation by vicia. Rhizobium species are usually defined as nitrogenfixing soil bacteria capable of inducing the. Introduction to rhizobia summary this module introduces the general role of microorganisms in the soil, and specifically the. Effectiveness of native rhizobium on nodulation and yield. Isabel revilla, in processing and impact on active components in food, 2015.
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